| To: fred hayes who wrote (6107) | 11/24/2004 4:24:57 PM | | From: scaram(o)uche | of 10345 | | | Iceberg used to discuss this sort of stuff, around here someplace. Maybe he can dredge pointers. I've got to focus on turkey and over hills and stuff(ing).
For years, I predicted that anti-TNF would be only modestly effective, as TNF was one of several inflammatory mediators. You couldn't be much closer to that project than I was. I also have plenty of expertise re. integrins, and I predict that anti-alpha4 will be only modestly effective for most autoimmune disorders.
;-)
J Pharmacol Exp Ther. 2004 Sep 23; [Epub ahead of print]
Selective inhibition of inflammatory gene expression in activated T lymphocytes: a mechanism of immune suppression by thiopurines.
Thomas CW, Myhre GM, Tschumper R, Sreekumar R, Jelinek D, McKean DJ, Lipsky JJ, Sandborn WJ, Egan LJ.
Mayo Clinic.
Azathioprine and 6-mercaptopurine are anti-metabolite thiopurine drugs that play important roles in the treatment of leukemia, and in the management of conditions requiring immunosuppression, such as inflammatory bowel disease. The biochemical pharmacology of these drugs suggests that inhibition of purine nucleotide formation through the 6-thioguanine nucleotide metabolites is their key molecular mechanism. However, it is unclear how these metabolites suppress immunity. We hypothesized that azathioprine produces a selective inhibitory effect on activated but not quiescent T lymphocytes. We first established a model system of T lymphocyte culture with azathioprine that produced pharmacologically relevant concentrations of 6-thioguanine nucleotides. Using genome-wide expression profiling, we identified a group of azathioprine -regulated genes in quiescent and activated T lymphocytes. Several genes involved in immunity and inflammation were selectively down-regulated by azathioprine in stimulated but not quiescent cells. Quantitative RT-PCR for 3 of these genes, tumor necrosis factor-related apoptosis-inducing ligand, tumor necrosis factor receptor superfamily member 7 and alpha4-integrin, confirmed down-regulated expression of transcript levels. Tumor necrosis factor-related apoptosis-inducing ligand protein expression was further studied and found to be inhibited by azathioprine, 6-mercaptopurine and 6-thioguanine, implying that the inhibitory effects of azathioprine on expression are mediated by 6-thioguanine nucleotides. These results therefore provide a previously unrecognized molecular mechanism for the immunosuppressive properties of thiopurine anti-metabolite drugs. |
| | Elan Corporation, plc (ELN) | Stock Discussion ForumsShare | Recommend | Keep | Reply | Mark as Last Read | Read Replies (1) |
|
